Figure 4

Bicalutamide reduced expression of prostate specific antigen, PSA, in intra-femoral PCSD1 tumors. Quantitative RT-PCR was performed on RNA extracted from the intra-femoral and subcutaneous PCSD1 tumors dissected from mice treated with bicalutamide (red bars) or vehicle control (blue bars). Quantitative RT-PCR was normalized to human-specific GAPDH expression levels. The same amount of RNA (1 ug) was used for cDNA synthesis and yield of RNA was similar per PCSD1 and LNCaP cell. The individual mouse numbers are shown on the horizontal axes ranked from low to high PSA level and are in the same order for all three Q-PCR plots, A-C. A.) PSA levels were higher in the IF than SC PCSD1 tumor cells. Bicalutamide treatment decreased PSA RNA expression in IF tumors but not SC tumors. Mann Whitney test was used to determine statistical significance (+ comparison of SC vehicle to IF vehicle treatment p<= 0.016; +comparison of SC bicalutamide to IF bicalutamide treatment p<=0.841; ◆ comparison of IF vehicle to IF bicalutamide treatment p<=0.008; ❍ comparison of SC bicalutamide to IF bicalutamide treatment p<= 0.73); B.) Bicalutamide increased AR expression in SC and IF tumors (same statistical comparison groups as in A.); C.) PSMA expression was unchanged in SC and IF tumors. Human GAPDH was used as an internal reference gene. Ratio of mean Cp of test to mean Cp of GAPDH reference gene is shown. Mann Whitney test was used to determine there was no statistically significant difference between any of the comparison groups. Error bars denote standard error.