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Figure 5 | Journal of Translational Medicine

Figure 5

From: Curcumin inhibits EMMPRIN and MMP-9 expression through AMPK-MAPK and PKC signaling in PMA induced macrophages

Figure 5

AMPK inhibitor mediated EMMPRIN, MMP-9 and MMP13 expression inhibition depends on the activation of MAPK pathway. A-C. Compound C, AMPK inhibitor, significantly inhibits EMMPRIN, MMP-9 and MMP13 expression in PMA induced THP-1 cells. Cells were pretreated with vehicle or Compound C (10 μM) for 1 h, followed by PMA for 48 h. The mRNA level of EMMPRIN, MMP-9 and MMP13 was determined by qPCR (A), and protein level was determined by Western blot (B) and quantified by densitometric analysis (C). Comp C indicates group treated with compound C; P + C indicates group treated with both PMA and compound C. D-E. Compound C inhibited the activation of MAPK pathway. Differentiated THP-1 cells were treated with indicated agents, and assayed by Western blot using indicated antibodies (D) and quantified by densitometric analysis (E). *P < 0.05 vs PMA group, **P < 0.05 vs CTL group.

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