Figure 4

PMA induced EMMPRIN, MMP-9 and MMP13 expression by macrophages is blocked by a specific ERK, p38 and/or JNK inhibitors. A-B. The addition of a specific ERK inhibitor (PD98059, 5 and 20 μM), p38 inhibitor (SB203580, 2.5 and 10 μM) completely blocks PMA-induced MMP-9 protein expression and activation, and p38-specific inhibitor showed weaker function. ERK1/2 and p38-specific inhibitor inhibitor significantly decreased EMMPRIN expression, whereas JNK specific inhibitor showed no inhibitory effect. C-D. ERK1/2, p38 and JNK-specific inhibitor at high dose showed remarkable inhibitory effect on MMP-13 expression. *P < 0.05 vs PMA group, #P >0.05 vs PMA group.