Figure 3

Curcumin inhibits JNK, ERK, and p38 phosphorylation and PKC pathway. A. Differentiated THP-1 cells were treated with indicated agents, and assayed by Western blot using indicated antibodies. Cells were pretreated with vehicle or curcumin at the indicated concentration (5–50 μM) for 1 h, followed by PMA for 48 h. B. Protein quantification was carried out by densitometric analysis. Normalized proteins of JNK, p-JNK, ERK, p-ERK, p38 and p-p38 were normalized based on the internal control β-actin. C. Expression of PKC-α and PKC-β1,cells were pretreated with vehicle or curcumin at the indicated concentration (5–50 μM) for 1 h, followed by PMA for 48 h. D. Densitometry measurements of protein analysis. The mean density values of PKC-αand PKC-β1 are expressed as ratios relative to that of β-actin. *P < 0.05 vs PMA group, **P < 0.05 vs CTL group.