Figure 2

Curcumin suppresses the activation of MMP-9 and chronic AMPK activation. A-B. Effect of curcumin on the activity of MMP-9 from cultured supernatant. THP-1 cells were pretreated with curcumin before stimulating with PMA (100 nM) for another 48 h. MMP-9 activities were detected by gelatin zymography assay (A), and the corresponding densitometric measurement was shown in B. Cur: curcumin. C-D. Effect of curcumin on total AMPK and phospho-AMPKα (Thr172). Differentiated THP-1 cells were treated with indicated agents and assayed by Western blot using indicated antibodies (C), and the corresponding densitometric measurement was shown in D. *P < 0.05 vs PMA group, **P < 0.05 vs CTL group.