Figure 5

Metabolic mechanism of adaptation. A) Flux arrest in glycolysis, citric acid and mycolic acid pathway; B) Activation of NAD pathway de novo as computed based on directional re-routing of metabolic fluxes; C) Alternate mechanism of ATP generation based on proton motive force; Mechanism: The activation of nadA ~ E operon lead to de novo biosynthesis of NADH, pool which is then reduced to NAD following the activation of the nuoA ~ Eoperon coding for NDH-I. This maintains the electron flow with proton translocation, which increases the potential difference across cell membrane, and can potentiate ATP production, thereby providing the necessary energy when challenged with antibiotic stress.