Mir-126-3p suppresses tumor growth and angiogenesis in vivo. (A) Overexpression of miR-126-3p significantly suppressed the growth of subcutaneous tumor growth. Growth curve and subcutaneous xenograft tumors removed were shown. (B) Subcutaneous xenograft tumors of lenvi-miR-126 or lenvi-NC were immunohistochemically stained for CD34. Tumors of lenvi-miR-126 displayed lower microvessel density. (C, D) HCC with lower miR-126-3p expression showed higher MVD, LRP6 and PIK3R2. MVD was evaluated based on CD34 staining and was determined from the three most intensely vascularized areas of each section at a magnification of 200×. The evaluation of LRP6 and PIK3R2 was performed at a magnification of 400×. The median of all 70 cases was chosen as the cutoff point for separating low miR-126-3p group (n = 35) from high miR-126-3p group (n = 35) in (C). *P < 0.05. **P < 0.01.