Flow cytometric characterization of macrophages, NK cells, and neutrophils in a representative sample of psoriatic adipose tissue. After exclusion of debris, doublets, and non-viable cells, cells were identified as follows: neutrophils = CD14-CD15+CD16+SSCHigranzyme B+, NK cells = CD14-CD15-CD16+/−CD56+/−SSClogranzyme B+, adipose tissue macrophages (ATM) = CD14+CD15-CD16-, which were further gated into 3 subpopulations based on HLADRII and CD206 expression. IL-1β and IL-8 intracellular staining is presented for the total ATM population. All cell populations are presented as percentages of viable cells except for IL-1β and/or IL-8 expressing ATM, and Granzyme B expressing neutrophils, which are reported as percentages of the parent population. Positive gating for each fluorochrome parameter was established using individual fluorescence minus one (FMO) controls.