Overexpression of RUNX2 induced expression of miR-10a and miR-10b and promoted cell migration and invasion. (A, B) MDA-MB-231 or MCF7 cells were transfected with 4 μg control pcDNA3.1 vector or pcDNA-RUNX2 plasmid for 72 hours. RUNX2, miR-10a or miR-10b expression levels were analyzed by qPCR. (C) RUNX2, HOXA1 and HOXD10 protein levels were assessed by Western blot analysis and normalized to actin. (D, E) Cell migration and invasion ability were assessed by Transwell assay or Matrigel assay, respectively. Black line: 20 μl, Red line: 100 μl (F, G). The quantification result was based on three independent experiments. *: p < 0.05, t-test.