RUNX2 silencing reduced the expression of miR-10a and miR-10b and inhibited cell migration and invasion. (A) Expression level of miR-10a and miR-10b in breast cancer cell lines were analyzed by qPCR analysis and normalized to U6 snRNA expression. (B) MDA-MB-231 cells were transfected with 25 μM control siRNA or RUNX2 siRNA for 72 hours. RUNX2, miR-10a or miR-10b expression levels were determined by qPCR analysis. (C) Expression levels of RUNX2, HOXA1 and HOXD10 proteins were assessed by Western blot analysis and normalized to actin. (D) Cell migration and invasion ability were assessed by Transwell assay or Matrigel assay, respectively as described in Material and Methods. Black line: 20 μl, Red line: 100 μl (E) The quantification result was based on three independent experiments. *: p < 0.05, t-test.