Figure 4
Effects of combined treatment with DCA and vemurafenib. A, B, Four melanoma cell lines (ED-013, ED-071, ED-117 and ED-196) were treated with DCA (1 mM), vemurafenib (50 nM for ED-071 and ED-117; 100 nM for ED-013 and ED-196) or the combination for 2 weeks. A, Crystal violet staining results representative of three independent experiments. B, Quantification of the data exemplified in A. C, Relative ATP levels in cells treated with DCA (IC50), vemurafenib (IC50) or the combination for 24 hours. B, C, Data represent means ± standard deviation of three independent measurements. One-way matched-samples ANOVA was used for statistical analysis and Tukey's HSD test was used to determine statistical significance (*p < 0.05; **p < 0.01).