miR-1 inhibited EMT and suppressed phosphorylation of ERK and AKT. (A) Western blot analysis was performed to detect the expression of epithelial cell marker E-cadherin and mesenchymal markers FN in SW480 cells transfected with miR-1. (B) The light microscopy was used to observe the change of cell morphology in SW480 cells after treatment with miR-1. The immunofluorescence (IF) assay was used to detect the expression of E-cadherin. (C) Phosphorylation levels of ERK and AKT were detected in SW480, HCT116 and SW620 cells transfected with miR-1 or anti-miR-1. (D) The immunosignal was quantified using densitometric scanning software, and relative protein abundance was determined by normalisation with total levels of ERK and AKT, respectively.