Figure 4

miR-1 altered global protein expression profiles and involved in several key biological processes. (A) 2-D DIGE images of SW480 cells transfected with miR-1 are shown. Proteins from cells transfected with control were labelled with Cy5. Proteins from cells transfected with miR-1 were labelled with Cy3. Internal standard proteins were labelled with Cy2. (B) Distribution of differentially expressed protein spots in merged images of the Cy-dye labelled images is shown. (C) Diagrammatic sketch showed major molecular functions exerted by miR-1 using Gene Ontology. (D) The histogram indicated main biological processes involved in miR-1 using Gene Ontology. (E) Both enlarged DIGE images and immunoblotting results of two candidate protein, identified as ARHGDIA and TAGLN, are shown.