miR-1 inhibited tumor growth and metastasis in nude mice. (A) The histogram indicates the increased expression of miR-1 in SW480 cells with miR-1 overexpression using qRT-PCR. (B) Cell proliferation was evaluated by CCK-8 assay between SW480/miR-1, which has stable overexpression of miR-1 and control SW480/miR-NC cells. (C) Representative figures and data of transwell assay for SW480/miR-1 and SW480/NC cells. Each bar represents the mean ± SD. The results were reproduced in three independent experiments. (D) Tumor cells were injected subcutaneously into the back of nude mice to evaluate cancerogenesis. Representative figure of tumors formed. (E) Tumor volume in the back of nude mice injected with SW480/miR-1 and SW480/NC cells was measured. The data of all primary tumors are expressed as mean ± SD. (F) Scatter plots of tumor volume derived from SW480/miR-1 and SW480/NC cells at 30 d after subcutaneous implantation. (G) Scatter plots of tumor weight derived from SW480/miR-1 and SW480/NC cells at 30 d after subcutaneous implantation. (H) Representative photographs of haematoxylin and eosin (H & E) and immunohistochemical staining of primary cancer tissues were shown (×400). N, necrotic lesion. (I) Number of liver and lung nodules per mice. The number of tumor nodules in individual mice was counted under the microscope. (J) Tumour cells were injected into nude mice through the spleen and tail vein to evaluate the lung and liver homing capacity of cells, respectively (H & E staining, ×200). The yellow crosses indicated tumor nodules in the liver and lung. T, tumor lesions.