Figure 7
Numbers of myeloid and plasmacytoid DCs in HSC grafts collected after mobilization with G-CSF and plerixafor (MZ). After the immunomagnetic removal of TCR-αβ+ T cells and CD19+ B cells, mononuclear cells contained within the graft were extensively characterized using mAbs directed against surface antigens selectively expressed on DC subpopulations [33]. DC subsets were also quantitated in aliquots of 10 normal BM samples from matched-sibling donors. Panels A and B depict the frequency and number of MDC1 and plasmacytoid CD303+ DCs, respectively, whereas panels C and D show the frequency and number of MDC2 and CD304+ plasmacytoid DCs, respectively. Comparisons among data sets were performed with the Mann Whitney U test for unpaired determinations or with the analysis of variance (ANOVA), as appropriate.