Figure 6

Numbers of monocytes and Slan-DCs in HSC grafts collected after mobilization with G-CSF and plerixafor (MZ). After the immunomagnetic removal of TCR-αβ⁺ T cells and CD19⁺ B cells, mononuclear cells contained within the graft were extensively characterized using mAb directed against surface antigens known to be expressed on monocytes and on pro-inflammatory Slan-DCs. Monocytes and Slan-DCs were also quantitated in normal BM samples from matched-sibling donors. The frequency and absolute numbers of CD14⁺CD16^- and CD14⁺CD16⁺ monocytes in TCR-αβ/CD19-depleted haploidentical grafts are depicted in panels A-D. The frequency and absolute number of Slan-DCs are shown in panels E-F. The green bars indicate median values. GM = ‘good mobilizers’; PM = ‘poor mobilizers’; HC = healthy control. Comparisons among data sets were performed with the Mann Whitney U test for unpaired determinations or with the analysis of variance (ANOVA), as appropriate.