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Figure 4 | Journal of Translational Medicine

Figure 4

From: Mobilization of healthy donors with plerixafor affects the cellular composition of T-cell receptor (TCR)-αβ/CD19-depleted haploidentical stem cell grafts

Figure 4

Mobilization of NK cells, NK-like T cells and monocytes with G-CSF and plerixafor (MZ). Panel A: Donor PBMCs were labeled with mAbs directed against CD3, CD16 and CD56 to enumerate classical NK cells, NK-cell subsets and NK-like T cells. A representative experiment illustrating the gating strategy for the analysis of NK cells and NK-like T cells is shown. After gating on CD3- events (P2), dot plots were used to visualize the reciprocal expression of CD16 and CD56 on NK-cell subsets. Panel B: The percentage of NK cells within the lymphoid gate (lymphs) is plotted. PB samples from 40 randomly selected donors were used for this analysis. GM = ‘good mobilizers’; PM = ‘poor mobilizers’; before = baseline samples; after = samples collected after HSC mobilization. Bars indicate the median value. Comparisons were performed with the Mann Whitney U test for paired determinations. Panel C: The percentage of each subset/total NK cells is plotted on y axes. Bars indicate the median value. Comparisons were performed with the Mann Whitney U test for paired determinations. Before = baseline samples; after = samples collected after HSC mobilization. Panel D: Percentage of NK-like T cells within the lymphoid gate. Bars indicate the median value. Comparisons were performed with the Mann Whitney U test for paired determinations. Panel E: Strategy to enumerate monocyte subsets in donors’ PB. Cells with a CD14++CD16- phenotype were considered to be conventional monocytes (Mo.); conversely, cells with a CD14+CD16+ (intermediate monocytes, Int.) or a CD14+CD16++ phenotype (non-conventional monocytes, NC) were referred to as CD16+ monocytes [9],[55]. No statistically significant differences emerged when comparing the frequency of monocytes before and after G-CSF ± MZ (panel F). WBC = white blood cells. The frequency of CD14+CD16- and CD14+CD16+ monocytes before and after HSC mobilization is depicted in panel G. Bars indicate median values. Comparisons were performed with the Mann-Whitney U test for paired determinations.

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