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Figure 3 | Journal of Translational Medicine

Figure 3

From: Mobilization of healthy donors with plerixafor affects the cellular composition of T-cell receptor (TCR)-αβ/CD19-depleted haploidentical stem cell grafts

Figure 3

T-cell cytokine secretion profile after mobilization with G-CSF and plerixafor (MZ). PBMCs from 18 randomly selected donors given G-CSF alone (G; n = 11) or G-CSF and plerixafor (MZ; n = 7) were stimulated with polyclonal activators in vitro and then fixed, permeabilized and stained with anti-IFN-γ/IL-17/IL-4 mAbs. Panel A: Percentage of cytokine-expressing CD4+ T cells at baseline and after mobilization. Bars denote the median value. Comparisons were performed with the Mann-Whitney U test for paired determinations. Panel B: A representative experiment for the enumeration of Th1, Th17 and Th2 CD4+ T cells is shown. The percentage of cells staining positively for each cytokine is indicated. Markers were set according to the proper isotypic control (not shown). Panel C: Percentage of IFN-γ-expressing CD8+ T cells at baseline and after mobilization. Bars denote the median value. Panel D: Percentage of IFN-γ-expressing CD4+ and CD8+ T cells in healthy donors assigned to the GM (G-CSF alone) and to the PM group (G-CSF + MZ). Bars denote the median value. Differences were not statistically significant.

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