Figure 1

CD34 ⁺ HSC mobilization algorithm. G-CSF was given subcutaneously at 12 μg/kg of body weight (divided into 2 doses, 12 hours apart) for 4 consecutive days (D). On day +4, circulating HSCs were enumerated by flow cytometry, as detailed in Materials and Methods. Panels A and B: If donors had ≤40 CD34⁺ cells/μL of blood on day +4 (and/or a predicted apheresis yield ≤12x10⁶ CD34⁺ HSCs/kg of recipient’s body weight) [27], a single dose of plerixafor (MZ) was administered subcutaneously (0.24 mg/kg of body weight), followed by HSC collection on day +5. If donors had >40 CD34⁺ cells/μL of blood on day +4 (and/or a predicted apheresis yield >12x10⁶ CD34⁺ HSCs/kg of recipient’s body weight), mobilization was continued with G-CSF alone and HSCs were collected on day +5 (panel C).