Figure 4

Effects of uremic toxins and specific siRNA treatment on NF-κB (A), AP-1 (B) and CREB-1 (C) activation by EMSA in HepG2 cells cultured for 12 h (n = 4 in each group). Normalized densitometric data of the NF-κB, AP-1, and CREB-1 bands obtained from the extracts of proteins with unclear interactions. N = negative control, A = controls, B = toxins, C = siRNA, and D = toxin + siRNA. Data are shown as the mean × standard deviation.