Figure 5

Reexpression of TFPI-2 in OSCC cells by gene transfer. (A) RT-PCR analysis of TFPI-2 expression in OSCC cells. Experiments were performed in triplicate and the results are shown as the changes in the expression relative to IOSE. Neg, untransduced cells; IG, cells transduced with a control lentiviral vector; FIG, cells transduced with a firefly luciferase-expressing lentiviral vector; TIG, cells transduced with a TFPI-2-expressing lentiviral vector. Error bars indicate standard deviations. (B) Western blot analysis of ectopic TFPI-2 overexpression in OSCC cells. ECM proteins were extracted from the cells for Western blot analysis and TFPI-2 isoforms with molecular weights of 27, 31, and 33 kDa due to different extents of glycosylation are indicated with arrows. Beta-actin was used as a loading control.