Figure 5

miR-10b modulates invasion factors RhoC, uPAR and MMPs expression via the target HOXD10 gene. (a) MHCC-97L cells were transfected with pcDNA3.1-miR-10b and control vector. MHCC-97H cells were transfected with anti-miR-10b and nonsense sequence. Then, HOXD10 protein levels were analyzed by Western blot. (b) In parallel, HOXD10 mRNA in the two cell lines treated as above was measured by real time RT-PCR. GAPDH served as internal control. (c) The protein expression levels of RhoC, uPAR, MMP-2 and MMP-9 were measured in the two cell lines treated as above was measured by Western blot, respectively.