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Figure 4 | Journal of Translational Medicine

Figure 4

From: miR-10b is overexpressed in hepatocellular carcinoma and promotes cell proliferation, migration and invasion through RhoC, uPAR and MMPs

Figure 4

The HOXD10 3′UTR is a target of miR-10b. (a) Upper panel, predicted duplex formation between human HOXD10 3′UTR and miR-10b. Lower panel, diagram of the luciferase reporter plasmids: plasmid with the full length wild-type HOXD10 3′UTR (pmirGLO-HOXD10-3′UTR) insert and plasmid with a mutant HOXD10 3′UTR (pmirGLO-HOXD10-3′UTR-mut) which carried a substitution of four nucleotides within the miR-10b binding site. (b) Luciferase activity assay demonstrates a direct targeting of the 3′UTR of HOXD10 by miR-10b. MHCC-97L and HepG2 cells were transfected with pcDNA3.1-miR-10b and pmirGLO-HOXD10-3′UTR or pmirGLO-HOXD10-3′UTR-mut. pRL-TK Renilla was used for normalization of transfection efficiency.

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