Figure 1

miR-10b is over-expressed in HCC tissues and cell lines. (a) The relative levels of miR-10b in sixty paired of HCC samples were measured by real-time quantitative RT–PCR, and the U6 small nuclear RNA was used as an internal control. Student’s t test was used to analyze the significant differences between the HCC and ANT tissues. (b) The expression of miR-10b in the five tissue groups. Benign tumor tissues and normal liver tissues were counted as control. One-way ANOVA was used to analyze the significant differences among the groups. (c) The expression of miR-10b in metastatic HCC (HHCC and LHCC) tissues and non-metastatic HCC (NHCC) tissues. The expression of miR-10b was calculated as the normalized ratio of the expression level of miR-10b in HCC tissues to the expression level of miR-10b in ANT tissues. Student’s t test was used to analyze the significant differences. (d) The relative levels of miR-10b in the seven HCC and three normal liver cell lines. One-way ANOVA was used to analyze the significant differences.