Figure 1

10058-F4 inhibits cell proliferation in ovarian cancer cells. SKOV3 and Hey cells were cultured for 24 hours and treated with F4-10058 as indicated doses in 96 well plates for 72 hours. Cell proliferation was assessed with MTT assay (A). 10058-F4 effect on long term growth in ovarian cancer cells was assessed through colony-forming assay (B). Western blotting results indicated F4-10058 inhibited c-Myc protein expression in a dose dependent manner after 36 hours treatment (C and D). *p < 0.05 and **p < 0.01, two-sided student t test.