The proportion of NKT cells in kidney. Lymphocytes were gated from all RBC-free single cell suspensions by FSC and SSC (not showed), CD3+ cells were gated in all lymphocytes, and NK1.1+ cells were selected in CD3+ lymphocytes. The proportion of NKT cells (CD3+NK1.1+) in kidney lymphocytes was calculated as the proportion of CD3+ cells in all lymphocytes multiplied by the NK1.1+ cells in CD3+ lymphocytes (A). The proportion of NKT cells (CD3+NK1.1+) in kidney tissue was significantly higher in Rapa group than in IR group and Sham group (B). Data are expressed as mean ± SD (n = 5).