Figure 3

The proportion of NKT cells in spleen. Lymphocytes were gated from all RBC-free single cell suspensions by FSC and SSC (not showed), then CD3⁺ cells were gated in all lymphocytes, and NK1.1⁺ cells were selected in CD3⁺ lymphocytes. The proportion of NKT cells (CD3⁺NK1.1⁺) in the spleen was calculated as the proportion of CD3⁺ cells in all lymphocytes multiplied by the NK1.1⁺ cells in CD3⁺ lymphocytes as the total quantity of splenocytes was manipulated to equal for each test (A). The proportion of NKT cells (CD3⁺NK1.1⁺) in spleen was significantly lower in Rapa group than in IR group (B). Data are expressed as mean ± SD (n = 5).