The proportion of NKT cells in spleen. Lymphocytes were gated from all RBC-free single cell suspensions by FSC and SSC (not showed), then CD3+ cells were gated in all lymphocytes, and NK1.1+ cells were selected in CD3+ lymphocytes. The proportion of NKT cells (CD3+NK1.1+) in the spleen was calculated as the proportion of CD3+ cells in all lymphocytes multiplied by the NK1.1+ cells in CD3+ lymphocytes as the total quantity of splenocytes was manipulated to equal for each test (A). The proportion of NKT cells (CD3+NK1.1+) in spleen was significantly lower in Rapa group than in IR group (B). Data are expressed as mean ± SD (n = 5).