Figure 5

Knockdown of Sp1 suppressed the clonogenicity and anchorage-independent growth of NPC cells. CNE2 and HNE1 cells transfected with either siSp1-1#, siSp1-2# or siNC were grow at low density for 10 days (A and B) or were plated onto 0.55% base agar (in RPMI with 5% FBS) in six-well plates for 14 days. For (A and B) Colonies were fixed with methanol and stained with 0.1% crystal violet in 20% methanol, and then were photographed and counted. All visible colonies were quantified. For (C and D), the colonies with the diameter bigger than 100 μm were counted. ** P < 0.01, *** P < 0.001.