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Figure 3 | Journal of Translational Medicine

Figure 3

From: Down-regulation of Sp1 suppresses cell proliferation, clonogenicity and the expressions of stem cell markers in nasopharyngeal carcinoma

Figure 3

Both impaired Sp1 activity and expression suppressed the cell viability of NPC cells. (A) Inhibition of Sp1 activity by MITA treatment decreased the cell viability of CNE2 and HNE1 cells. Twenty-four hours after seeding in 96-well plates, CNE2 and HNE1 cells were treated with 100 nM MITA. The cell viability was determined by MTT assay at the indicated times. ** P < 0.01 and *** P < 0.001. (B) Sp1 was completely abolished in CNE2 and HNE1 cells. After transfection with siSp1-1#, siSp1-2# or siNC for 72 h, cells were subjected to Western blot analysis for Sp1. GAPDH was used as an internal control. (C) Silencing of Sp1 impaired the cell viability of CNE2 and HNE1 cells. After transfection with siSp1-1#, siSp1-2# or siNC for 48 h, CNE2 and HNE1 cells were seeded into 96-well plates (2000/well) and incubated for the indicated times. The cell viability was determined by MTT assay at the indicated times. *** P < 0.001.

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