Figure 1

Expression of Sp1 is higher in NPC cell lines than the normal primary NPECs. Sp1 protein and mRNA levels were analyzed by Western blot (A and B) and qRT-PCR (C) in the normal primary NPECs (NPECw and NPEC01), and NPC cell lines (HNE1, HONE1, CNE2 and C666-1). These results were reproducible in three independent experiments and the representative immunoblots were shown in A. Both the protein and mRNA levels of Sp1 were normalized to GAPDH.