A microRNA profile of human CD8+ regulatory T cells and characterization of the effects of microRNAs on Treg cell-associated genes

Table 1 Relative regulatory T cell-associated gene expression for CD8 ⁺ CD25 ⁺ /CD8 ⁺ CD25 ⁻ by individual qPCR (SYBR Green)

Relative Treg-associated gene expression
Gene	IL2RA	FOXP3	CTLA-4	CCR4	GARP	IL10	TGF-B
CD8 + CD25+/CD8 + CD25-	65 ± 1,414	11,5 ± 4,9	3.19 ± 1,73	13.43 ± 4,69	13,66 ± 4,12	4.86 ± 2,68	4.57 ± 2,61
Gene	CD28	ICOS	FOXO1	HELIOS
CD8 + CD25+/CD8 + CD25-	0,05 ± 0,019	0,342 ± 0,125	0,18 ± 0,078	0,288 ± 0,13

Gene mRNA levels were evaluated using quantitative RT-PCR of CD8⁺CD25⁺/CD8⁺CD25⁻ T cells. Data represent the mean ± SD of five independent experiments, each done in triplicate.

ISSN: 1479-5876