Figure 6

MicroRNA specific activities. (A) In primary human Treg cells, miR-335 specifically regulates FOXP3 expression. Relative miR-335 and FOXP3 expression in CD8⁺CD25⁺ Treg cells transduced by lenti-miR-335 compared with CD8⁺CD25⁺ Treg cells transduced by lenti-miR-Ctrl, as determined by relative qRT-PCR. (B) In primary human Treg cells, miR-9 and −155 specifically regulate CTLA-4 expression. Relative miR-9, miR-155 and CTLA-4 expression in CD8⁺CD25⁺ Treg cells transduced by lenti-miR-9 or lenti-miR-155 compared with CD8⁺CD25⁺ Treg cells transduced by lenti-miR-Ctrl, as determined by relative qRT-PCR. (C) In primary human Treg cells, miR-24 and −335 specifically regulate GARP expression. Relative miR-24 and GARP expression in CD8⁺CD25⁺ Treg cells transduced by lenti-miR-24 compared with CD8⁺CD25⁺ Treg cells transduced by lenti-miR-Ctrl, as determined by relative qRT-PCR. Relative miR-335 and GARP expression in CD8⁺CD25⁺ Treg cells transduced by lenti-miR-335 compared with CD8⁺CD25⁺ Treg cells transduced by lenti-miR-Ctrl. as determined by relative qRT-PCR. CD8⁺CD25⁻ T cells were considered to be non-Tregs; *p < 0.05, **p < 0.01, ***p < 0.001 (as determined by Student's t-test).