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Figure 6 | Journal of Translational Medicine

Figure 6

From: A microRNA profile of human CD8+ regulatory T cells and characterization of the effects of microRNAs on Treg cell-associated genes

Figure 6

MicroRNA specific activities. (A) In primary human Treg cells, miR-335 specifically regulates FOXP3 expression. Relative miR-335 and FOXP3 expression in CD8+CD25+ Treg cells transduced by lenti-miR-335 compared with CD8+CD25+ Treg cells transduced by lenti-miR-Ctrl, as determined by relative qRT-PCR. (B) In primary human Treg cells, miR-9 and −155 specifically regulate CTLA-4 expression. Relative miR-9, miR-155 and CTLA-4 expression in CD8+CD25+ Treg cells transduced by lenti-miR-9 or lenti-miR-155 compared with CD8+CD25+ Treg cells transduced by lenti-miR-Ctrl, as determined by relative qRT-PCR. (C) In primary human Treg cells, miR-24 and −335 specifically regulate GARP expression. Relative miR-24 and GARP expression in CD8+CD25+ Treg cells transduced by lenti-miR-24 compared with CD8+CD25+ Treg cells transduced by lenti-miR-Ctrl, as determined by relative qRT-PCR. Relative miR-335 and GARP expression in CD8+CD25+ Treg cells transduced by lenti-miR-335 compared with CD8+CD25+ Treg cells transduced by lenti-miR-Ctrl. as determined by relative qRT-PCR. CD8+CD25 T cells were considered to be non-Tregs; *p < 0.05, **p < 0.01, ***p < 0.001 (as determined by Student's t-test).

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