Figure 7

Blockade of platelet adhesion to inflamed human aortic (HAEC) and human umbilical endothelial vein endothelial cells (HUVEC) by F11R siRNA: inhibition by silencing of the F11R gene. Transfection of HUVEC and HAEC was conducted by using either the non-targeting siRNA or the F11R targeting F11R siRNA, as detailed in the Material and Methods section. Following transfection, both HAEC and HUVEC were pretreated with a combination of cytokines TNFα (100 u/ml) and IFNγ (200 u/ml) for 24 hrs. Afterwards, suspensions of either non-activated or collagen-activated platelets (as detailed in the Material and Methods section) were applied unto monolayers of the inflamed ECs, and the adhesion of platelets to the cytokine-treated ECs was monitored. The values represent the adjusted means ± SEM for the number of platelets bound to the ECs/per well from 5 separate experiments. * P < 0.05.