Figure 2

The expression of F11R mRNA in human endothelial cells (ECs) exposed to proinflammatory cytokines TNFα and IFNγ: dose response. Endothelial cells, HAEC and HUVEC in culture, were treated with different concentrations of TNFα (0.5 to 1000 pM; 1 to 2000 units) and IFNγ (0.1 - 100 nM; 3.4 - 3448 units/ml) for 12 hrs at 37°C. Real-time PCR was performed three times in triplicate for each time point. Values represent the mean ± SEM. * P < 0.05. Significant differences in F11R mRNA observed at the indicated concentrations of cytokines in comparison to levels of F11R mRNA measured in the absence of cytokines.