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Figure 6 | Journal of Translational Medicine

Figure 6

From: Protein kinase CK2α is overexpressed in colorectal cancer and modulates cell proliferation and invasion via regulating EMT-related genes

Figure 6

Inhibition of CK2α reversed the nuclear translocation of β-catenin and altered EMT-related genes expression. Reversal of EGF-induced nuclear translocation of β-catenin occurred in LoVo cells transfected with CK2α-specific siRNA (A), treated with EGF (100 ng/ml) for 2 h, and stained for immunofluorescence with β-catenin antibody (red) and DAPI (blue). (B) Western blot was used to detect the expression levels of CK2α, E-cadherin, β-catenin, vimentin and the transcription factors snail1 and smad2/3 in cells transfected with CK2α-specific siRNA. (C) One week later, in LoVo cells treated with emodin (40 μmol/l, 50 μmol/l and 60 μmol/l), the expressions of E-cadherin, β-catenin and vimentin were detected by western blot analysis. GAPDH expression was used as a loading control.

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