Figure 4

CK2α inhibition induced G0/G1 phase arrest. (A) LoVo cells were transfected with CK2α-specific siRNA or nonspecific siRNA, stained with propidium iodide (PI), and monitored by flow cytometry to determine the cell cycle phase distribution. (B) Comparison of the percentage of cells in each phase of the cell cycle between LoVo cells transfected with CK2α-specific siRNA and nonspecific siRNA. Columns, mean of three independent experiments; bars, SD. *P < 0.01. (C) CK2α, p53, p21, C-myc and GAPDH expression in cells transfected with CK2α-specific siRNA was detected by western blot analysis.