Figure 4
Cellular function tests of MTSS1 in oesophageal cancer cell lines. (A) In vitro cell growth assay. (Left panel) Over-expression of MTSS1 significantly reduced cell growth rate. (Right panel) Suppression of MTSS1 expression levels enhanced cell growth rate. (B) In vitro cell matrix adhesion assay. (Left panel) Cell adhesive ability was dramatically enhanced through over-expression MTSS1 in KYSE150. (Right panel) A reduction in the adhesive nature of KYSE510 was observed through the knockdown of MTSS1 expression. (C) In vitro motility assay. (Left panel) Over-expression of MTSS1 significantly inhibited the motile nature of oesophageal cancer cells. (Right panel) Knockdown of MTSS1 expression dramatically enhanced cell migration. (D) In vitro invasion. (Left panel) The presence of MTSS1 within KYSE150 significantly suppressed the invasive capacity. (Right panel) Knockdown of MTSS1 promoted the invasiveness of KYSE510. Each cell line was tested in triplicate, three independent experiments were done. Bars, SD.