Figure 1
From: Body fluid derived exosomes as a novel template for clinical diagnostics
Characterization of amniotic fluid, urine and saliva derived microvesicles. (A) One representative example of microvesicles for amniotic fluid, saliva and urine was analyzed after sucrose density fractionation by Western blot analysis. (B) Isolated exosomes were adsorbed to latex beads and stained for the exosomal markers CD9, CD24 and ADAM10 followed by phycoerythrin-conjugated goat anti-mouse immunoglobulin G and FACS analysis. The negative control represents beads stained with the secondary antibody alone. The gray curve represents the autofluorescence of unstained beads. Note that the staining intensity is proportional to the amount of antigen on the exosomal surface.