Temporal changes in Kupffer cells following LA treatment of selected liver metastases. Groups of mice (n >5) had two tumors LA treated and tissues were collected at various time points post ablation. Control mice had sham LA performed (LA probe was not activated). F4/80 positive areas were calculated using image pro plus software. Values are expressed as mean arbitrary units ± SEM. Panel a; black bar, Kupffer cells at the tumor host interface distant from sham treated tumors; grey bars, Kupffer cells at the tumor host interface distant from ablated tumors. P > 0.05 compared to sham control at all time points. Panel b; black bar, F4/80 staining in liver tissue of sham treated mice; grey bars, F4/80 staining in liver tissue distant from ablated tumor site. * P < 0.05 compared to sham control.