Temporal changes in CD3+ T cells following LA treatment of selected liver metastases. Groups of mice (n >5) had two tumors LA treated and tissues were collected at various time points post ablation. Control mice had sham LA performed (LA probe was not activated). Panel a temporal changes in CD3+ T cells at the tumor host interface; grey bars = LA treated tumors, white bars = distant tumors and black bars = sham controls. Panel b temporal changes in CD3+ T cells in the liver parenchyma post LA treatment. Black bars = sham controls, grey bars = LA treated. Results are expressed as mean number of cells/mm2 ± SEM. * and # P < 0.05, * * P < 0.01 compared to controls.