Immune responses following LA treatment of liver tissue. Groups of mice (n >5) had LA treatment in the liver and tissue was collected at various time points post ablation as indicated. Control mice had sham LA performed (LA probe was not activated). Panels a and b, sham treated liver sections at three days post treatment. Panels c and d, liver sections distant from the site of ablation at three days post treatment. Panels e and f, ablated liver sections 3 days post treatment depicting the injury front. Original magnification 200x. Panel g, temporal changes in CD3+ T cells at the injury front following LA treatment expressed as mean number of cells/mm2 ± SEM, * P < 0.05, compared to control. Panel h, enumeration of IFNγ secreting splenocytes at 3 days post liver LA treatment, expressed as mean number of spots/million splenocytes ± SEM, ** P < 0.001, compared to control.