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Figure 6 | Journal of Translational Medicine

Figure 6

From: A predicted protein, KIAA0247, is a cell cycle modulator in colorectal cancer cells under 5-FU treatment

Figure 6

Changes in intracellular localization according to KIAA0247 expression and 5-FU treatment. Immunofluorescent staining in the p53-null HCT116 cells (HCT116 p53-/-). A) shLuc cells (200 ×) and B) overKIAA0247 cells (200 ×) stained for cellular KIAA0247 using diluted anti-KIAA0247 antibody. Secondary antibody, R-phycoerythrin (PE) -conjugated goat anti-rabbit antibody; DNA counterstaining, 4",6" diamidino-2-phenylindole (DAPI). LM, light microscope images; Merge, merged images from PE and DAPI. White arrowhead, cells with nuclear accumulation of KIAA0247; bars, 25 μm.

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