Table 1 Proteins, peptides and transitions selected from LC-MS/MS spectra and the corresponding parameters for MRM verification of plasma expression levels.
Proteins | Peptides | Transitions precursor ion [M+H]*-> product ion | Retention time (min) | dwell time (min) | Fragmentor energy (kV) | Collision energy (kV) |
|---|---|---|---|---|---|---|
ApoA1 | DYVSQFEGSALGK | 701.1->532.4 | 34 | 100 | 200 | 20 |
701.1->661.4 | 25 | |||||
701.1->808.5 | 30 | |||||
Hemopexin | EVGTPHGIILDSVDAAFICPGSSR | 829.8>650.3 | 44 | 100 | 200 | 25 |
829.8>992.3 | 30 | |||||
829.8>909.7 | 27 | |||||
Angiotensin preprotein | ADSQAQLLLSTVVGVFTAPGLHLK | 822.8->664.4 | 62 | 100 | 200 | 20 |
822.8->877.1 | 25 | |||||
822.8->816.7 | 22 |