Figure 2

Designed hybrid Antp-TPR peptide demonstrates selectivity for cancer-cell killing. (A) The indicated cancer or normal cell lines were incubated with Antp-TPR peptide. (B) TPR peptide needs to be combined with Antp, the cell-penetrating peptide, to have a selective cell-killing effect. (C, D) Mutation analysis of TPR peptide examining its effect on cell killing. The indicated cell lines were incubated with Antp-TPR mutant 1 (C), in which highly conserved Arg and the subsequent amino acid, Ile, in the TPR peptide were replaced with Ala, or mutant 2 peptide (D), in which last three amino acids of TPR, Tyr-Phe-Lys, were replaced with three Gly residues to disrupt the helix structure of TPR. All cell viability was analyzed after 72 h incubation of test peptides as described Materials and Methods section. Data represent the mean ± SD from experiments performed in triplicate.