Methylation levels of the

Table 3 Cox analysis of the influence of LINE- 1 methylation on OS of stage IIIC CM patients

LINE1 CpG site	# events/# patients*	Extent methylation^†	HR^‡	95% CI; P value	HR_cont.^§	95% CI; P value
CpG1	13/21	<25.68	1**		1.51	1.06-2.16; 0.02
	17/21	≥25.68	1.57	0.76-3.24; 0.22
CpG2	12/21	<27.26	1		1.60	1.02-2.52; 0.04
	18/21	≥27.26	2.12	1.01-4.44; 0.04
CpG3	11/21	<40.46	1		1.49	1.06-2.09; 0.02
	19/21	≥40.46	2.63	1.21-5.69;. 0.01

* number of patients who died (# events) and total number of patients in the group (# patients) are reported;
† Patients were divided according to the % of methylation of the specified CpG site being <or ≥ the median % methylation measured in the examined patients' population;
^‡ Cox proportional hazard method was used to examine the effect of LINE-1 methylation on OS. Results were presented as Hazard Ratios (HR) with corresponding 95% Confidence Intervals (CI);
^§ LINE-1 methylation was also evaluated as continuous variable. The HR value is that of the LINE-1 methylation relative to an increase of 10%;
** set as reference.

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