Figure 1

Effects of vemurafenib, metformin or the combination on cell proliferation and viability. a) Example of growth curves of BRAF^V600E and NRAS^Q61 mutant melanoma cell lines. BRAF^V600E cell lines included M263 (synergistic) and M308 (antagonistic). As representatives for NRAS^Q61 mutants M207 (synergistic) and SKMEL173 (antagonistic) are shown. The black line represents the data obtained with vemurafenib treatment, the blue line with metformin, and the red line with combination treatment. b) Combination index for the combination of 5 μM vemurafenib and 10 mM metformin of all melanoma cell lines tested. The green cell lines harbor BRAF^V600E, red shows NRAS^Q61 mutant cell lines, and black the wild type cell line M257.