Figure 3

VX-680 inhibits activation of Aur-A and induces monopolar spindle in NB4-R2 cells. (A) VX-680 inhibited phosphorylation of Aur-A at Thr288 in NB4-R2 cell line. Cells were incubated with increasing doses of VX-680 for 24 hr and 48 hr and subjeceted to Western blot with antibodies of pAur-A and GAPDH. (B) The morphology of mitotic spindle was shown by immunofluorescence staining with anti-α-tubulin antibody and anti-Aur-A antibodies. Microtubules were stained as green, Aur-A protein as red, and nucleus as blue.