Figure 2

Transduction and enzyme activities of PEP-1-SOD1 and PEP-1-CAT fusion proteins in H9c2 cells. (a-c): Time and dose-dependent transduction of PEP-1-SOD1. Control or 2 μM SOD1 was added into the culture medium for 15~60 min (a-Short time course); 0.5~2 μM PEP-1-SOD1 or control SOD1 was added to the culture medium for 1 h (b-dose dependent); or cells pretreated with 2 μM PEP-1-SOD1 were incubated for different times (1~48 h) (c-longer time course). Western blots were performed using anti-His antibody. (A~C): Time and dose-dependent transduction of PEP-1-CAT. 2 μM PEP-1-CAT or control CAT was added into the culture medium for 15~360 min (A-shorter time course); 0.5~2 μM PEP-1-CAT or control CAT was added to the culture medium for 6 h (B-dose-dependent); or cells pretreated with 2 μM PEP-1-CAT were incubated for different times (6~72 h) (C-longer time course). Western blots were performed using anti-His antibody. (d~f): Enzymatic activity of PEP-1-SOD1. (D~F): Enzyme activity of PEP-1-CAT. Results are mean ± SD, n = 5, *P < 0.01, ^#,$ P < 0.05 vs control (CTL) group in each individual set of experiments.