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Figure 6 | Journal of Translational Medicine

Figure 6

From: Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells

Figure 6

Suppression of Bcl-2 with siRNA induced apoptosis and increased susceptibility to DNR. KG1a and Kasumi-1cells were transfected with siRNA Bcl-2 or siRNA control for 24 h. (A) Bcl-2 protein levels were determined by Western blotting assay. (B) Cells were stained with Annexin V/PI to analyze apoptotic cell populations. The graph displays the means ± SD of three independent experiments. (C) KG1a and Kasumi-1 cells were transfected with siRNA Bcl-2 or siRNA control for 24 h, and then treated with DNR (0.2 μg/ml) for 48 h. Cells were stained with Annexin V/PI to analyze apoptotic cell populations. The bar represents mean ± SD of three independent experiments. ** p < 0.01, *** p < 0.001 (compared with control).

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