Figure 4

Curcumin decreased Bcl-2 mRNA and protein levels and caused the loss of MMP. KG1a, Kasumi-1 and U937 cells were exposed to different concentrations of curcumin for 24 h. (A) The effects on Bcl-2, c-IAP-1, and XIAP mRNA levels were determined by RT-PCR. (B) The effect on Bcl-2 protein levels was determined by Western blotting assay. Three independent experiments were performed with similar results, and representative data are shown. (C) MMP was estimated by flow cytometry showing decrease in the red to green fluorescence ratio. The results shown are representative of three independent experiments. The bar represents mean ± SD of three independent experiments.